Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
Extracellular lipase production from Bacillus subtilis using agro-industrial waste and fruit peels
Haniya Mazhar1, Naaz Abbas2, Zahid Hussain3, Amir Sohail4, Syed Shahid Ali4*
ABSTRACT
This study was planned for the production of lipases by solid-state fermentation (SSF) using agro-industrial wastes as substrate. A total of 14 bacterial strains were isolated, screened and compared for lipase production. Among the evaluated strains, Bacillus subtilis strain NL-39 was selected on the basis of best lipase production. The maximum enzyme activity (34.93 U/ml) was shown by B. subtilis using SSF with soya bean meal. The lipase production by other low cost agro-industrial waste, including sunflower meal, wheat bran, oat bran, rice bran and sugar cane bagasse was 23.83, 12.17, 10.40 10.00 and 16.23 U/ml, respectively. The lipase production by B. subtilis using peels of different fruits, including banana, orange, water melon and melon as carbon source, was 27.17, 21.37, 10.57 and 8.43 U/ml, respectively. The corn cob produced 12.27 U/ml while waste oils of various industries produced 16.17 U/ml (Shan oil), 13.67 U/ml (automobile), 13.37 U/ml (unbranded waste cooking oil) and 6.03 U/ml (Sitara oil) of enzyme activity when used as substrate for lipase production. It is concluded that among the agro-industrial wastes, fruit peels and waste oil samples used in this study, the maximum lipase production was observed with soya bean meal, banana peel and Shan oil waste, respectively.
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