Infection of Galleria mellonella larvae by Steinernema affine and production of infective juveniles
Infection of Galleria mellonella larvae by Steinernema affine and production of infective juveniles
A. M. Rahoo1, 2, †, T. Mukhtar3, B. A. Bughio4, S. R. Gowen1 and R. K. Rahoo5
ABSTRACT
The information regarding relationship between nematode dosage and infective juveniles (IJ) production are
available for Steinernema affine. Therefore, in the present study the effect of inoculation doses of S. affine and
extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater
emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction
trays. In Baermann extraction trays, the maximum emergence of IJ of S. affine was observed at an inoculation dose
of 400 IJ followed by the dose of 50 IJ. The minimum emergence was found with an inoculation dose of 100 IJ. On
the other hand, in case of White traps, the maximum emergence of IJ was recorded with doses of 100 and 50 IJ. The
minimum emergence of IJ was observed with a dose of 200 IJ. The relationships between inoculation doses and
emergence of IJ from the cadavers for both the methods were found to be non-significant.
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June 2017
Pakistan Journal of Nematology, Vol. 35, Iss. 1, Pages 03-111
