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Bacterial Expression and Purification of Antigenic Recombinant Protein Encoded by Hepatitis Delta Virus Antigen of Pakistani Isolate

Bacterial Expression and Purification of Antigenic Recombinant Protein Encoded by Hepatitis Delta Virus Antigen of Pakistani Isolate

Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3

1Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan
2Centre of Applied Molecular Biology, University of the Punjab, Lahore, Pakistan
3University of Peshawar, Peshawar, Pakistan.
 
* Corresponding author: iram_mali2005@yahoo.com

ABSTRACT

HBV is a global burden and co-infection of HBV/HDV causes additional risks and mortality rate. Currently, there is no vaccine available for HDV, therefore, treatment of patients with dual infection is indeed a challenge for clinicians. A timely and accurate diagnosis is critical for treatment in this regard. Expression and purification of HDAg, which is the only protein of HDV of the local isolate is the primary objective of the study. This antigenic recombinant HDAg protein can be useful for both vaccine development and as a diagnostic marker of HDV. After determination of HDAg antigenic region of HDV and its amplification, the fragment was cloned in bacterial expression vector. The expression of recombinant protein was checked by SDS-PAGE and after the purification of protein by using Ni-affinity column. antigenic protein was confirmed with Western Blot analysis. The study demonstrates the expression and purification of HDAg recombinant protein of HDV local isolate in the bacterial expression system. The purified protein has potential to be useful as an antigen in Diagnostic ELISA and as a vaccine candidate.

 

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Pakistan Journal of Zoology

October

Vol. 54, Iss. 5, Pages 2003-2500

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