Molecular, clinico-pathological and sero-diagnosis of LSDV in cattle at Sharkia and Fayoum Governorates
Molecular, clinico-pathological and sero-diagnosis of LSDV in cattle at Sharkia and Fayoum Governorates
Nashwa M. Helmy1, Ahmed S. Ahmed2, and Zeinab3 Y. Mohamed
ABSTRACT
Background: A total of 30 biopsy samples from cutaneous nodules were obtained from infected animals at different stages during the course of the Lumpy skin disease (LSD), and (100) Peripheral blood samples without anticoagulant were drawn from apparently healthy non vaccinated cattle against LSDV and (100) serum samples were drawn from cattle 4 weeks post vaccination with local attenuated sheep pox virus vaccine located in Sharkia and Fayoum governorates. Methods: Lumpy skin disease virus (LSDV) was isolated from skin biopsies collected from clinically infected cattle. The virus was isolated on MDBK cell line and identified by agar gel precipitation test (AGPT) and indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against LSDV. Further identifications were carried out by polymerase chain reaction (PCR) and clinco-pathological investigation. Results: The results showed that 11/30 biopsies were positive by AGPT, 19/30 by IFAT and 30/30 by PCR. While results of sero-diagnosis showed that 45/100 from apparently healthy non vaccinated cattle and 68/100 from vaccinated cattle were positive by SNT respectively and in general 90/200 of tested cattle give protective antibody titer, while 23/200 gave non protective titer and 87/200 have no antibody againest LSDV. The results of clinco-pathological revealed highly significant increase in ALT, AST, ALP, GGT, urea and uric acid, while the level of total protein, albumin and calcium showed significant decrease and non-significant reaction in creatinine and non-organic phosphorus in infected cattle. The results of antioxidant both malondialdehyde (MDA) and catalase enzyme (CAT) showed significant increase while level of gloutathion (GSH), total antioxidant capacity (TAC) and gloutathion peroxidas (GPX) showed significant decrease in infected cattle. Conclusion: Sero-survey, conventional techniques and PCR assay should be applied besides clinco-pathological for any cases with skin lesions as early as possible to diagnosis and apply adequate control measures. The results encountered in the present study revealed that cattle infected with LSD exposed to strong oxidative stress so recommended to use antioxidants in infected animals during treatment.
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