Molecular Detection of Entamoeba histolytica in Human and Cattle
Molecular Detection of Entamoeba histolytica in Human and Cattle
Mohammed Jawad Kadhim*, Qasim Jawad Amer
ABSTRACT
The purpose of this research is to determine how common Entamoeba histolytica is in Babylon City, Iraq, among both people and animals. One hundred samples were taken from humans and one hundred from cattle around the province, for a grand total of 200 fecal samples. PCR and microscopy were performed to assess the genetics and diversity of the Entamoeba histolytica. The ITS1 region of the parasite’s small subunit rRNA gene was amplified using a PCR. in conjunction with microscopy. The microscopy results revealed the existence of parasite cysts and trophozoites in both human and cattle fecal samples. The PCR results indicated that the genus Entamoeba was detected in 43% (43/100) of the human fecal samples (27 in male and 16 in female). Moreover, the results showed that the high frequency of E. histolytica was in children less than 5 years old (48.6%) as compared to patient from 5-10 years and patient more than 10 years where a rate of 38.2% and 41.4% was observed, respectively. For cattle, PCR indicated 59% positivity based on the ITS gene. A total of 13 were positive in male and 46 were positive in female. On the other hand, the high frequency (63.2%) of E. histolytica was recorded in animals from 2 years old and above as compared with those less than 2 years (56.5%). In conclusion, the molecular approaches employed in the present investigation shown a remarkable degree of sensitivity and specificity.
Keywords | Entamoeba histolytica, Cattle, PCR, Babylon, Human and Diarrhea
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November 2024
Vol. 12, Sp. Iss. 1
