Frozen-Thawed Quality of Bull Semen after Combined Supplementation of Docosahexaenoic Acid and Alpha Linolenic Acid into Tris based Semen Extender
Frozen-Thawed Quality of Bull Semen after Combined Supplementation of Docosahexaenoic Acid and Alpha Linolenic Acid into Tris based Semen Extender
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
ABSTRACT
This the study was conducted to find out the effect of the addition of alpha linolenic acid (ALA) and docosahexaenoic acid (DHA) on frozen-thawed quality of bull semen. Twenty-four ejaculates (8 from each bull) were collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm characteristics such as sperm motility, viability, morphology, membrane integrity, and acrosome integrity were decreased in treated groups. DHA concentration was increased in all supplemented groups. Whereas ALA decreased in all groups except at 5ng/ml DHA was increased linearly by increasing supplementation of fatty acids. In conclusion, combination of ALA and DHA did not improve frozen-thawed bull sperm parameters when compared to control.
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December 2017
Vol. 49, Iss. 6, Pages 1937-2341
