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Antibiotic Residues of Penicillin and Oxytetracycline in Yoghurt: One Health Needs Paradigm Shift, Legislation and Policy Formulation in Pakistan

Antibiotic Residues of Penicillin and Oxytetracycline in Yoghurt: One Health Needs Paradigm Shift, Legislation and Policy Formulation in Pakistan

Muhammad Ali Raza1*, Aneela Zameer Durrani2, Bilques Bano3, Muhammad Muddassir Ali4, Nazia Rubab5, Syed Tasadak Mehdi6, Muhammad Wasim Iqbal7, Kumay Hassan Akhtar8, Aqeel Raza9, Ujala Fatima Shan10, Muhammad Aftab11

1BE and OE, HQs, Federal Government, Islamabad, Pakistan
2Clinical Medicine Department, University of Veterinary & Animal Sciences (UVAS), Lahore, Pakistan
3School Education Department, Government of the Punjab, Pakistan
4Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences (UVAS), Lahore, Pakistan
5Population Welfare Department, Government of the Punjab, Pakistan
6Children Hospital and Institute of Child Health, Multan, Pakistan
7Massey University, New Zealand
8Graduate School of Chinese Academy of Agricultural Science, Beijing, China
9Veterinary Science and Technology, FVS, Chulalongkorn University, Bangkok, Thailand
10Department of Clinical Medicine, University of Veterinary & Animal Sciences (UVAS), Lahore, Pakistan
11Department of Clinical Medicine, University of Veterinary & Animal Sciences (UVAS), Lahore, Pakistan
 
Corresponding author: Muhammad Ali Raza

ABSTRACT

Yoghurt is considered a nutritional food rich in proteins and other essential minerals. Poor quality milk having Antibiotic Resistance resides threat to one health. Antibiotic Resistance is the main issue that affects the livestock, humans and environment. Antibiotic Resistance occurs due to the use of excessive amount of antibiotics for the growth and development of livestock. 90 samples of each weigh about 50 grams have been collected from Lahore and brought to University of Veterinary and Animal Sciences, Lahore in a Laboratory and applied HPLC technique on samples and found residues of Penicillin and oxytetracycline in different percentages (11% and 9%, respectively) and percentage of both combine Penicillin and oxytetracycline is 3%. In a yoghurt sample, the highest penicillin was found as 13.95 µg/Kg, the lowest penicillin was detected as 7.07 µg/Kg, the highest oxytetracycline was observed as 306.73 µg/Kg, the lowest oxytetracycline was figured out as 197.77 µg/Kg. The result is statistically significant and against the Null Hypothesis. It shows that significant difference has been estimated among all of the positive samples of yogurt. We found the commonly used antibiotics (Penicillin and oxytetracycline) in routine food i.e., in yoghurt samples collected from open market of Lahore, District of Punjab Province, Pakistan. 
 
Novelty Statement | The detection of antibiotics (ABs) residues of penicillin and oxytetracycline in yoghurt through HPLC have been detected for the first time in Lahore, Punjab, Pakistan as a research study.

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Punjab University Journal of Zoology

June

Vol.39, Iss. 1, Pages 01-134

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