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An Evaluation Study for Chlorophyll Estimation Techniques

An Evaluation Study for Chlorophyll Estimation Techniques

Kawa A. Ali1*, Sazar S. Noraldeen2 and Arshad A. Yaseen2, 3

1Field Crop Department, College of Agricultural Engineering Sciences, Salahaddin University, Erbil, Iraq; 2Department  of Horticulture, College of Agricultural Engineering Sciences, Salahaddin University, Erbil, Iraq; 3University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management, Institute of Horticultural Science, H-4032 Debrecen, Boszormenyi Street 138, Hungary. 

 
*Correspondence | Kawa A. Ali, Field Crop Department, College of Agricultural Engineering Sciences, Salahaddin University, Erbil, Iraq; Email: kawa.ali@su.edu.krd 

ABSTRACT

A field experiment was conducted to evaluate the efficacy of two destructive and two nondestructive chlorophyll estimation techniques for two vegetable crops (aroma tomato Solanum lycopersicum L. and green pepper Capsicum annuum L.) in three different environments (open field, lath house and plastic house) to indicate the best method of chlorophyll estimation. After 80 days from sowing chlorophyll estimation was implemented first by nondestructive methods using SPAD (SPAD-502 Plus Konica Minolta Tokyo, Japan) and atLEAF chlorophyll meter (at-LEAF FT-GREEN-LLC Willington, DE, USA) instruments, then the same leaves were cut and chlorophyll estimation were done by destructive methods using two organic solvents ( 80% acetone and 100% ethanol). Results demonstrate significant differences between SPAD and atLEAF data for tomato and pepper plants where higher levels with atLEAF instrument comparing to SPAD chlorophyll meter. Higher chlorophyll levels were recorded in open field plants comparing to lath and plastic houses plants. Chlorophyll a content was related to carotenoid levels in both destruction methods. There was positive correlation between SPAD and atLEAF. Nondestructive methods could be used as an alternative method for destructive methods. 

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Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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