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Studies on Antimicrobial Properties of Neem (Azadrichta indica) Seed Oil

Studies on Antimicrobial Properties of Neem (Azadrichta indica) Seed Oil

Hailemariam Assefa1*, Tesfahun Dagnaw2 

1Department of Chemistry, Mekdela Amba University, Ethiopia; 2Department of Chemistry, Wolaita Sodo University, Ethiopia.

*Correspondence | Hailemariam Assefa, Department of Chemistry, Mekdela Amba University, Ethiopia; Email: 


This study aimed to determine the in vitro antimicrobial activities of Neem seed oil by r disk diffusion method. Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and Streptococcus pyogenes were used as tested bacterial species whereas Yeast, Mold and Dandruff were selected as tested fungi. The effect was seen high on Staphylococcus aureus, while the Klebsiella pneumoniae was less affected by any of the used extracts. Methanolic extract was more active than petroleum ether and ethyl acetate extract on all three tested fungal species. The oil was obtained by soxhlet extraction method. The maximum oil yield using petroleum ether was found 18.4% at a temperature of 70oC and a minimum yield of 13.5% at 30oC while at the same operating condition ethyl acetate and methanol resulted a maximum yield of 17.0% and 16.5% and a minimum of 12.0% and 13.0% respectively. So petroleum ether is a good solvent to afford high yield in this work. The oil was analyzed by GC-MS and result in the identification of the compounds, (Palmitoleic acid methyl ester, Palmitic acid methyl ester, Linoleic acid methyl ester, Oleic acid methyl ester, Stearic acid methyl ester and etc) was obtained. In the present study various physical and chemical characteristics have been also studied. The results also indicated that the oil extracted from Neem seed is the most acidic and non edible.

Keywords | In vitro antimicrobial, Paper disk diffusion, Neem seed, Soxhlet extraction, GC-MS, non-edible, Identification of the compounds 

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Advances in Animal and Veterinary Sciences


Vol. 12, Iss. 3, pp. 392-585


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