Efficacy of Chitosan Nanoparticle Transplantation on Regeneration of Acute Spinal Cord Injury in Dogs Model
Efficacy of Chitosan Nanoparticle Transplantation on Regeneration of Acute Spinal Cord Injury in Dogs Model
Ahmed Kadhim Munahi1* , Hameed A. Al-Timmemi2
ABSTRACT
The most common cause of persistent neurological diseases is spinal cord damage. Unfortunately, the vast majority of spinal cord injury treatments are ineffective. The current study is aimed to apply chitosan nanoparticles on spinal cord regeneration in dogs. For this objective, sixteen healthy mongrel dogs were used. They were divided into two equal groups (n=8) at random. All dogs underwent dorsal laminectomy and left lateral hemisection at the level of the second lumbar vertebra. In the control group, the hemisections were treated with 0.2 mL of phosphate buffer saline. Chitosan nanoparticles were applied to the chitosan group with hemisection stie. From the first week to the completion of the experiment 16 weeks following surgery, the open field locomotor scale, which comprised gait, proprioceptive posture, and nociception pain, was utilized to assess motor and sensory gains. After 16 weeks of postoperative testing, the motor and sensory reflexes in the treated group were substantially different (p<0.05) from the control group. The neurological recovery scale (normal gate to leap) in chitosan group was at 15 weeks post treatment while in control group, the animals didn’t retain to that state until end of experiment. Chitosan nanoparticles group histopathological examinations revealed reduced cavitation, orientation of regenerative nerve fibers in white matter, increased number of regenerative neuron cells in grey matter, increased angiogenesis, and minimal scar tissue formation at the injured spinal cord site. In conclusion and based on clinical and histological data, chitosan nanoparticles could accelerate and promote regeneration of the injured spinal cord.
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