Lumpy skin disease is circulating among sheep pox vaccinated cattle in Egypt 2017
Lumpy skin disease is circulating among sheep pox vaccinated cattle in Egypt 2017
Akram I. Aboelkhair1, Ayman H. El-Deeb2, Momtaz A. Shahin1 and Hussein A. Hussein2
ABSTRACT
Background: Lumpy skin disease is an infectious viral disease caused by Lumpy skin disease virus
(LSDV) affecting cattle causing skin lesions. It`s a member of Poxviridea family, genus Capri-pox,
and closely related to sheep and goat pox. In Egypt, the protection from lumpy skin disease (LSD)
among cattle population was carried out using sheep poxvirus vaccine. Last year (2016-2017) revealed
many cases of LSD in sheep pox vaccinated cattle in Egypt.
Objective: In this study LSDV was isolated from sheep pox vaccinated cattle showing LSD signs to
confirm LSD viral infection even in sheep pox vaccinated cattle.
Methods: 130 Nodular samples were collected from 150 vaccinated cattle showing signs of LSD,
Then Molecular detection of LSDV with PCR using G-protein-coupled chemokine receptor (G-PCR)
gene primer revealed positive result for LSDV, So classical Isolation of LSDV was started by
inoculation of nodules on Chorioallantoic membrane (CAM) 9-11 day old SPF eggs, then adaptation
on Madin-Derby bovine kidney cell line ( MDBK ); confirmation of LSD isolated virus was done by
using Electron microscopy (EM) on suspension of cell culture passage of the virus, Serological
identification was done by serum neutralization test ( SNT ) using reference antisera which confirm
presence of LSDV . By nucleotide sequences and genetic characterization was conducted on (G-PCR)
gene segment of these isolates.
Results: 93 samples were positive for LSDV by using conventional PCR direct from nodular lesion, 4
positive samples were inoculated on CAM showed characteristic pock lesion, formerly after passaging
of samples on MDBK cell line revealed characteristic cytopathic effect of LSDV, as well as tissue
culture suspension were positive using conventional PCR . For antigenic identification SNT were
applied. Sequencing of positive sample reveled identity of LSDV by targeting G-protein coupled
chemokine receptor gene (GPCR) which confirm that there is no genetic changes in LSDV.
Conclusion: phylogenetic analysis of LSDV at summer 2017 revealed that recent isolates has no
genetic difference compared by other isolates from Egypt or Africa during previous few years, as
LSDV affected sheep poxvirus (SPV) vaccinated cattle,
To share on other social networks, click on any share button. What are these?
January 2019
Vol. 5, Pages 1-141
