Comparative Analysis of Serum Adiponectin and its Genetic Polymorphisms in Metabolic Syndrome and Healthy Group-an Observational Study
Comparative Analysis of Serum Adiponectin and its Genetic Polymorphisms in Metabolic Syndrome and Healthy Group-an Observational Study
Uzma Zafar1*, Zaima Ali1, Ambreen Tauseef2 and Saba Khaliq3
ABSTRACT
The present study was executed to determine the association of adiponectin gene polymorphisms rs1501299 +276 G>T and rs266729 -11377 C>G with metabolic syndrome and to compare serum adiponectin levels in its polymorphic genotypes. It was an observational study conducted at University of Health Sciences, Lahore. Study approval was granted by institutional Review/Ethical Board. Informed consent was taken from all the participants. The venous blood sample was taken after an overnight fast of 8-10 h. Blood was secured for DNA analysis and biochemical tests. Adiponectin rs266729(-11377 C>G) and rs1501299(+276G>T) genotypes were determined by ARMS PCR. Serum adiponectin levels were measured by ELISA. The current study included 200 metabolic syndrome cases and 200 healthy subjects. The minor allele ‘G’ of -11377 C>G [p=0.013; OR (CI)=1.47(1.08-1.999)] and the major ‘GG’ genotype of +276 G>T [(p=0.027; OR(CI)=1.56(1.05-2.32)] were associated with the increased risk of metabolic syndrome. The major ‘CC’ genotype of -11377 C>G was associated with the decreased risk [(p=0.004; OR (CI)=0.55(0.36-0.83)] of metabolic syndrome. Serum adiponectin was higher significantly in the wild genotype (CC) of -11377 C>G in cases and controls. Serum adiponectin correlated inversely with blood pressure, waist circumference and fasting serum glucose; and it exhibited positive correlation with serum high density lipoproteins in the controls while no significant relation was found in the cases. The major “GG’’ variant of adiponectin +276 G>T was associated with the increased risk whereas the major “CC” variant -11377 C>G was associated with the decreased risk of metabolic syndrome. It is concluded that the serum adiponectin levels were significantly less in the minor “GG” variant of -11377 C>G in metabolic syndrome as well as in the healthy group.
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