Isolation and Characterization of Plant Growth Promoting Fungi from the Rhizosphere and Bulk Soil of Abelmouschus esculentus L.
Isolation and Characterization of Plant Growth Promoting Fungi from the Rhizosphere and Bulk Soil of Abelmouschus esculentus L.
Imad Ahmad Noor1, Abdullah1, Ihteram Ullah2*, Kashif ur Rahman4, Imtiaz Ahmed1 and Habib Ur Rahman3
ABSTRACT
Hormones are chemical substances that exist in trace amounts in definite tissues and control a variety of processes correlated to development, metabolism, and reproduction in the target tissue. The chemical generated by plants are known as phytohormones. During current study we isolated five fungi of which three strains were isolated from the rhizosphere soil of A. esculentus L. while the remaining strains were isolated from the bulk soil in the plant vicinity. The result revealed that the rhizosphere and bulk soil fungi have the potential to promote okra growth. It was found that both the rhizosphere and bulk soil had traces of Salicylic acid (SA). The quantities of Salicylic acid (SA) were higher in the bulk soil than the rhizosphere soil. The isolated fungi also released salicylic acid, sugars and proteins as evident from detection in their culture filtrate. The fungal isolates obtained from bulk soil including ZIB1 (Zarab Imad Bulk strain 1) and ZIB8 (Zarab Imad Bulk strain 8) also released proteins in their culture filtrates. Inoculation of fungus strains ZIR1 (Zaryab Imad rhizosphere strain 1), ZIR4 (Zaryab Imad rhizosphere strain 4), ZIR6 (Zaryab Imad rhizosphere strain 6), ZIB1, and ZIB8 enhanced the host growth attribute in term of root and shoot length. Fresh and dry weight of seedlings was also enhanced when associated with fungi. The fungal strains didn’t influence each other growth when grown side by side on the agar plate. It concluded that both the fungi can be used as bio-inoculants in combination for plant growth and development. Based on the current study the above mentioned fungal strains are suitable and can be utilized to enhance the growth of okra plant.
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