Determination of Antibiotic Resistance Pattern and Molecular Characterization of Corynebacterium pseudotuberculosis from Lymph Node Samples of Sheep and Goats in Ethiopia
Determination of Antibiotic Resistance Pattern and Molecular Characterization of Corynebacterium pseudotuberculosis from Lymph Node Samples of Sheep and Goats in Ethiopia
ABSTRACT
Corynebacterium pseudotuberculosis consists of non-motile and pleomorphic rods. The frequent rout of entry of C. pseudotuberculosis into the body of animal is in punctures and scratches of the skin. It results in the development of caseous abscesses in external and internal lymph nodes. Therefore, the following were the objectives of this research: to identify and isolate bacteriologically, to characterize molecularly C. pseudotuberculosis from the external nodes of lymph of sheep and goats, and to determine multiple drug resistance of C. pseudotuberculosis in sheep and goats in Halal Food Industries, Modjo, Ethiopia. A total of 450 animals (100 sheep and 350 goats) were examined during antemortem and postmortem inspections. Bacteriological isolation and identification of the collected lymph nodes were performed and then confirmed by PCR. Then, the antibiotic resistance profile was revealed. Seven (17.7%) of goat isolates from Oromia, 5 (12.5%) from Southern Nations, nationalities and peoples regional states and 4 (10%) sheep isolates from Oromia were evaluated as positive for C. pseudotuberculosis in molecular diagnosis. C. pseudotuberculosis was moderately susceptible to gentamicin (68.75%) and highly susceptible to penicillin G (100%), doxycycline (100%), and ciprofloxacin (100%) had been observed. In conclusion, risk associated factors and seroepidemiology of caseous lymphadenitis in sheep and goats should be investigated in Ethiopia.
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