Epidermal Growth Factor (EGF) and Basic Fibroblast Growth Factor (FGF2) Profiles in Follicular Fluid, Maturation Media and Embryo Culture of Bali Cattle
Epidermal Growth Factor (EGF) and Basic Fibroblast Growth Factor (FGF2) Profiles in Follicular Fluid, Maturation Media and Embryo Culture of Bali Cattle
Erni Damayanti1,2, Herry Sonjaya2*, Sudirman Baco2, Hasbi Hasbi2, Ekayanti Mulyawati Kaiin3
ABSTRACT
A study on growth factors in Bali cattle embryo culture media in vitro has never been carried out and this study aims to measure the concentration of FGF2 and EGF in follicular fluid, maturation media and Bali cattle embryo culture media. The study used ovaries that came from slaughterhouse and were oocytes mature before fertilization using semen from one bull, oocytes are fertilized then cultured for 48 hours. Embryo grouping based on cell count was conducted after 48 hours of culture and was recultured for 48 hours. Follicular fluid, oocyte maturation media and embryo culture media were collected using a disposable syringe and centrifuged, then supernatant in the collection, stored and frozen with -20 oC and growth factor concentrations were measured using the ELISA method. The results showed that there were 228 developing embryos (41%) after 48-hour of culture with 2-, 4-, 8-, and 16-cell division. EGF concentrations in the medium of 2-cell to 16-cell ranged from 0.8539 ng/mL-0.2838 ng/mL. The concentration of FGF2 in follicular fluid is 0.629 ng/mL, while the concentration of FGF2 in maturation media, culture media 2 cells, 4 cells, 8 cells and 16 cells respectively, namely 0.032 ng/mL, 0.015 ng/mL, 0.011 ng/mL, 0.009 ng/mL and 0.008 ng/mL. In conclusion, EGF concentration was found more in follicular fluid and culture media and was not found in maturation media. while FGF2 was found to be higher in follicular fluid than in maturation media and embryo culture media. The EGF and FGF2 concentration decreases as the embryonic development stage increases.
Keywords | Bali cattle embryos, Basic fibroblast growth factor (FGF2), Epidermal growth factor (EGF), In vitro culture
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September 2023
Vol. 11, Iss. 9, pp. 1411-1596
