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Chlorpyrifos and Endosulfan Mixture Induced DNA Damage and Nuclear Anomalies in RBCs of Labeo rohita Assessed Through Comet and Micronucleus Assay

Chlorpyrifos and Endosulfan Mixture Induced DNA Damage and Nuclear Anomalies in RBCs of Labeo rohita Assessed Through Comet and Micronucleus Assay

Huma Naz1*, Sajid Abdullah2, Tanveer Ahmed3*, Khalid Abbas2, Syed Qaswar Ali Shah1, Muhammad Rizwan1, Fareeha Latif4, Adnan Ahmad Qazi1, Muhammad Adeel Hassan5

1Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
2Department of Zoology, Wildlife and Fisheries, University of Agriculture Faisalabad, Pakistan
3Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology, Rahim Yar Khan, Pakistan
4Institute of Zoology, Bahauddin Zakariya University Multan 
5Department of Parasitology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, Pakistan
 
Corresponding authors: Huma Naz and Tanveer Ahmed
 dr.humanaz98@gmail.com, tanvirahmeduaf@gmail.com

ABSTRACT

Abstract | Insecticides are widely used to control the insect damaging food and cash crops as well as the fruit plants. However, terrestrial and aquatic biodiversity is directly affected by overuse of insecticides. Present study was conducted to find out the genotoxic effect of insecticides on DNA damage and nuclear anomalies in RBCs of Labeo rohita after exposure to chlorpyrifos+endosulfan (CPF+END) by using Comet and Micronucleus Assay. Twenty fingerlings of L. rohita was kept in LC50 concentration of CPF+END, (1.95±0.02 μgL-1 for 96 h) for 5 days after acclimatization. The blood sample of 5 fish was collected after 24, 48, 72 and 96 h to see the DNA damage and nuclear anomalies. Results of the current study revealed that damaged nuclei (DN%) and genetic damage index (GDI) in RBCs of L. rohita exposed to insecticides mixture was increased throughout the experiment. Similarly, the frequency of micronuclei (MN) and other nuclear anomalies (NAs) were also increased as the duration of exposure passed. It is concluded that exposure of fish to insecticides may cause considerable genetic damage to the fish. The both Comet and MN Assay are good biomarkers to identify the insecticides pollution. The results of our study can be utilized in environmental risk evaluation and in bio-monitoring approaches. 
 
Novelty Statement | This research sheds light on the genotoxicity and environmental risks associated with endosulfan and chlorpyrifos pesticides in freshwater ecosystems and offers new insights into the potential synergistic effects of these pesticides on aquatic organisms.

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Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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