Biological Characterization and Pluripotent Identification of Cartilage Stem/Progenitor from Peking Duck
Biological Characterization and Pluripotent Identification of Cartilage Stem/Progenitor from Peking Duck
Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*
ABSTRACT
A group of cells in cartilage tissue with stem cell characteristics may be ideal seed cells for cartilage defect repair. In previous studies, stem/progenitor cells from articular cartilage have been isolated and identified in humans, horses, and bovines, but rarely in ducks. We isolated and cultured cartilage stem/progenitor cells (CPSCs) from the articular cartilage of 14-day-old Peking duck embryos using the fibronectin adhesion method. The morphology of CPSCs was observed under an inverted microscope. Based on the growth curve test, we found that the growth mode of the cells was “S” type growth. The clonogenic assay was performed to determine the clonogenic efficiency CPSCs. The expression of cell surface markers was detected by immunofluorescence, flow cytometry, and RT-PCR, and it was found that the CPSCs positively expressed FGFR-3, collagen type II, CD44, CD73, CD90, vimentin, Notch1, CD105, and CD106, but negatively expressed the haematopoietic stem cell surface marker CD34. Furthermore, CPSCs were successfully induced to differentiate into adipocytes, osteoblasts, and chondrocytes, showing that they have an in vitro differentiation ability. These findings provide a theoretical and experimental basis for cell transplantation therapy in tissue engineering.
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