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Occurrence, Antimicrobial Susceptibility and Phylogroups of Escherichia coli O157:H7 Isolated from Food Outlets in Some Touristic Cities in Egypt

Occurrence, Antimicrobial Susceptibility and Phylogroups of Escherichia coli O157:H7 Isolated from Food Outlets in Some Touristic Cities in Egypt

Tarek Refaay, E.A. Elshafiee, Hayam A. Mansour and Maha A. Sabry*

 

Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Giza Square, PO Box 12211, Cairo, Egypt

 
* Corresponding author: profdrmahaas@cu.edu.eg

ABSTRACT

Foodborne illnesses are frequently caused by Escherichia coli (E. coli). E. coli O157 is regarded as a potentially harmful cause of gastrointestinal disorders associated with consumption of foods with animal origin. Therefore, this study was conducted to determine the presence of E. coli O157:H7 in food outlets in some touristic cities in Egypt. For this purpose, 648 samples including raw chicken meat, cooked chicken meat, raw beef meat, cooked beef meat, food handlers and equipment swabs were collected from 54 food outlets in some touristic cities in Egypt. E. coli O157 was 1.1% (7/648) and 1.2% (5/432) in all examined samples and food samples respectively. Cooked chicken samples were the most contaminated with E. coli O157:H7 with an overall prevalence of 1.9% (2/108). The highest prevalence of E. coli O157:H7 (8.3%) isolates was recovered from raw chicken and cooked beef meat in Hurghada Governorate followed by Luxor Governorate (6.3%). There is no E. coli O157:H7 isolates were identified in Sharm El Sheikh and Aswan governorates. All E. coli O157:H7 isolates (100%) showed resistance to ampicillin (AMP), cefixime, ciprofloxacin and cotrimoxazole. Multidrug resistance (MDR) was observed among all E. coli O157:H7 isolates. All E. coli O157:H7 isolates harbor the eae gene with complete absence of stx1 gene. The most prevalent phylogroup among the E. coli O157:H7 strains was B2 identified in raw and cooked beef and cooked chicken, collected from Luxor, Hurghada, and Alexandria governorates, respectively. Whereas, D phylogenetic group E. coli O157:H7 was only found in raw chicken sample collected from Hurghada Governorate. In conclusion, the detection of pathogenic MDR E. coli O157:H7 in food samples, food handlers and food equipment in some touristic cities in Egypt poses a serious risk to public health. Therefore, it is recommended to focus on identifying practices which increase the risk of food contamination, and on implementing measures to improve the sanitary conditions in the food outlets in touristic cities.

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Pakistan Journal of Zoology

February

Pakistan J. Zool., Vol. 56, Iss. 1, pp. 01-501

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