Sero Survey on current status of Foot and Mouth Disease in some Egyptian Governorates 2013 - 2015
Sero Survey on current status of Foot and Mouth Disease in some Egyptian Governorates 2013 - 2015
Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3
ABSTRACT
Foot-and-mouth disease (FMD) is a highly contagious viral disease affecting both domesticated
and wild cloven-hoofed animals worldwide and it is a disease with high economic importance. In
Egypt, FMD has taken as enzootic form and many outbreaks had occurred since 1950 and
onwards. FMDV serotype (O) was the most prevalent until serotype (A) appeared in 2006 then
during April and May 2012, six outbreaks of FMD serotype (SAT 2) were reported in Egyptian
governorates. This study based on evaluation of vaccinated animals by detection of antibodies
against serotypes of FMDV (A),(O) and (SAT-2) by (SNT) as well as by analysis of negative
Non Structural Protein sera by solid phase competitive ELISA for differentiation between natural
infected and vaccinated animals by detection of Non-Structral protein of FMDV by priocheck
test. Five hundred sera were collected from vaccinated cattle and buffalo from five Egyptian
governorates (Two governorates represent Delta region: El-Gharbia – Kafr El Sheikh , Two
governorates represent upper Egypt: El-Fayoum – El-Menya , and one governorate represents
central : El-Giza). The sera collected from diseased and apparent healthy animals. Results of
Serum Neutralization Test were 260 of cattle sera out of 280 and 167 of buffalo sera out of 220
were +ve by SNT. Priocheck test was applied on two hundred cattle and buffalo sera from the
positive sera of SNT its results indicated that 48 sera were positive(29 for cattle and 19 for
buffalo) , sertotyping for positive sera were applied with statistical results showed no significance
difference between serotypes in governorate , species and percent of inhibition . Solid Phase
Competitive ELISA was applied for the negative 152 sera samples to measure the titer of
antibody against strains A,O, SAT-2 with high titer of antibody against serotype (A) was 87 ,
serotype (O) was 72 and serotype (SAT-2) was 53 . Matching between SNT and ELISA was
applied for negative sera samples.
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January 2015
Vol. 12, Pages 1-125
