Genomic Homology and Mixed Interactions of Baculoviruses Infecting the Egyptian Cotton Leaf Worm Spodoptera Littoralis In Vivo and In Vitro
Genomic Homology and Mixed Interactions of Baculoviruses Infecting the Egyptian Cotton Leaf Worm Spodoptera Littoralis In Vivo and In Vitro
Omaima Khamiss
ABSTRACT
Baculovirus genomes from different virus species generally exhibit a considerable degree of structural diversity. However, some sequenced baculovirus genomes from closely related viruses are structurally very similar and share overall nucleotide sequence identities in excess of 95%. This work focuses on the comparative essential basis studies to better know complex interactions between baculoviruses infecting the pest in question (Spodoptera littoralis) in relation to stability or mechanism of infection in the same host multiple nucleopolyhedroviruses (MNPVs) Spli MNPV, and granulovirus SpliGV. Mixed infection of the tow viruses was carried out in vivo and in vitro, showing clearly that the mixture with Spli GV enhance the effect of Spli NPV when it was introduced 24hr prior to NPV or simultaneously: In addition to' protein profiles by SDS-PAGE, TEM electron microscopy ultrathin sections and hybridization dot blots, gel transfer to follow the probable mechanism especially with the high percentage of genome homology that was over 66% with one of five tested restriction enzymes. These observations suggest an important possible role for recombination in the early evolution and biological characteristics of these two viruses.
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July 2012
Vol. 9, Iss. 1
