Isolation and characterization of frankia from root nodules of Coriaria nepalensis, a non-legume in the Murree forests
Isolation and characterization of frankia from root nodules of Coriaria nepalensis, a non-legume in the Murree forests
Mohammad Arif Chaudhry and Ashraf Hassan Chaudhry
ABSTRACT
Frankia CN5 isolated from Coriaria nepalensis Wall.
was found comparable to some of its previously reported
strains in morphological and physiological
characteristics. The isolate produced reddish brown to
whitish brown pigmentation on solid medium. Though Qmod
medium proved to be the best for isolation but it showed
maximum growth on P+N and tween-80 media, in 28 days. In
the absence of combined nitrogen, the strain CN5 fixed
nitrogen showing acetylene reduction assay (ARA) and
was found highly sensitive to higher concentration of
NaCI (500mM in yeast extract-dextrose medium). This
strain gave the infective and effective results for
nodulation on the root system of C. nepalensis. The
light microscopy revealed the occurrence of seperate and
branched hyphae bearing sporangia and vesicles.
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March 1992
Vol. 42, Iss. 1
