Preparation and Evaluation of Inactivated Oil Emulsion Vaccine Against Swollen Head Syndrome in Chickens
Preparation and Evaluation of Inactivated Oil Emulsion Vaccine Against Swollen Head Syndrome in Chickens
Hala M. El-Makaky*•, Sherif, N.A.;Fekria El-Bordiny* and Taha, M.M.*
ABSTRACT
In the present study, inactivated single swollen head syndrome oil emulsion vaccine (SHSV) was prepared and evaluated. SHSV was successfully propagated on the yolk sac of 5-7 days of SPF embryonated chicken eggs. The virus was harvested 48-72 hours post inoculation. The titre of the virus reached 1062 EID50ml. One hundred and fifty chickens were divided into 3 equal groups. The first group was vaccinated with the prepared SHS vaccine, 2nd group two was vaccinated with imported SHS vaccine, while the third group was kept as non-vaccinated control. Evaluation of the prepared vaccine was done by measuring both cell-mediated and humoral immunity. The results of both T-lymphocyte transformation and ELISA tests revealed that the prepared vaccine induced good protection and has good potentiality for use in chicken in the Egyptian poultry industry.
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