LncRNALOXL1-AS1 Regulates the Proliferation and Apoptosis of Ovarian Cancer Cells by Targeting miR-761
LncRNALOXL1-AS1 Regulates the Proliferation and Apoptosis of Ovarian Cancer Cells by Targeting miR-761
Dajun Su1, Tao Deng2* and Mingshui Xie2
ABSTRACT
The aim of this study was to investigate the molecular mechanism of lncRNALOXL1-AS1 regulating the proliferation and apoptosis of ovarian cancer cells by targeting miR-761. Ovarian cancer cells SKOV-3 and normal ovarian cells FTE187 were divided into groups according to the experimental procedures, and were transfected negative, respectively. The expressions of lncRNALOXL1-AS1 and miR-761 in ovarian cancer cells were measured by qRT-PCR (quantitative real-time PCR). LncRNALOXL1-AS1 targeting miR-761 was detected by dual luciferase reporter gene assay. CCK8 and flow cytometry were used to detect the proliferation and apoptosis of ovarian cancer cells in each group. Compared with normal ovarian epithelial cell FTE187, the expression of LOXL1-AS1 in ovarian cancer SKOV-3 cells was significantly up-regulated and the expression of miR-761 was significantly down-regulated; compared with the miR-NC group, the expression level of miR-761 in SKOV3 cells was significantly increased in the miR-761 group; after LOXL1-AS1 interference, SKOV3 cell proliferation and colony formation were significantly decreased. miR-761 expression was up-regulated and apoptosis rate was increased. The biological behavior of the LOXL1-AS1+ miR-inhibitor NC group was similar to that of the LOXL1-AS1siRNA group, and miR-inhibitor treatment could reduce the above effects of LOXL1-AS1 on cells. LncRNALOXL1-AS1 can regulate the proliferation and apoptosis of ovarian cancer cells, and the mechanism may be related to the regulation by targeting miR-761. Intervention of LOXL1-AS1could inhibit proliferation and promote apoptosis of ovarian cancer cells in vivo and in vitro. Further studies showed that the mechanism of LOXL1-AS1 action on ovarian cancer cells is related to the regulation by targeting miR-761. The regulation of LOXL1-AS1 may be an effective approach for the treatment and prevention of ovarian cancer.
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