This project was a 2×2 factorial design executed for the evaluation of optimum lipid/protein level in pelleted diets for Labeo rohita (Initial weight; 2.87±0.01g). Four pelleted diets varying in their lipid/protein levels i.e. 7.5/25, 9.5/25, 7.5/30 and 9.5/30% were formulated and hand fed for 90 days to four groups of 10 fish each. Results of the present study showed that L. rohita attained significantly higher average wet weights, fork and total lengths of 4.79±0.04g, 66.63±0.04mm and 74.67±0.04mm, respectively due to 9.5/30% lipid/protein diet (D4). None of the dietary lipid/protein levels in pelleted diet had a significant impact on either the condition or the survival of fish. Significantly higher (p<0.05) specific growth rate (SGR) and feed efficiency (FE) for L. rohita was also due to 9.5/30% lipid/protein diet (D4). The pelleted diets upon fatty acid analysis showed almost complete absence of linoleic acid, α-linolenic acid, ecosapentanoic acid (EPA) and docosahexanoic acid (DHA). However, these fatty acids were found to be present in the flesh of L. rohita at the end of the experiment. The sum of saturated fatty acids (SFA) was higher as compared to the sum of monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) in L. rohita flesh. Regardless of the feeding regimes there existed a higher proportion of n-3 fatty acids as compared to n-6 fatty acids in the flesh of L. rohita. Ratio of n-3/n-6 fatty acids in L. rohita flesh was significantly higher (p<0.05) due to 9.5/25% (D2) lipid/protein level in pelleted diet. Moisture contents of L. rohita varied significantly among the treatments, showing an inverse relationship with body fats. The feeding regime D4 (9.5/30% lipid/protein level) gave fish the highest flesh proteins i.e. 19.17±0.03%. The carcass lipid were generally higher in fish groups fed lower protein level whereas the fish groups fed higher protein level fetched comparatively lower body lipids. Body ash contents of L. rohita varied slightly among the treatments showing no obvious significant influence of varying lipid/protein levels.
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