Improving the Efficiency of Campylobacter spp. Isolation from Livestock and Poultry in Ukraine
Improving the Efficiency of Campylobacter spp. Isolation from Livestock and Poultry in Ukraine
Natalia Shchur1,2*, Tetiana Mazur1, Orest Katsaraba3, Ihor Halka2, Ludmila Shalimova2, Larisa Moskalenko4, Tetiana Ponomariova-Herasymiuk4, Maksym Lusta4, Vitalii Nedosekov1
ABSTRACT
This article presents research on the spread of Campylobacteriosis pathogens in Ukraine, revealing infection rates of 26.3% in broilers, 3.33% in turkeys, 55% in pigs, and 8.33% in cattle. In this research of the study, 33 isolates of Campylobacter spp. were identified for the first time, representing 23.9% infection, among which Campylobacter coli accounted for 75.8% and Campylobacter jejuni for 24.2%. Notably, up to 70% of poultry isolates were identified as Campylobacter coli. To improve the indication system, methods were modified by reducing cultivation time, simultaneously using selective nutrient media of various compositions, and employing both commercial materials and those prepared from components. Additionally, a comparative assessment of two isolation methods was conducted using a model of 138 samples of cecal contents from poultry (n=106) and animals (n=32) from farms in Vinnytsia, Volyn, Kyiv, and Cherkasy regions. 97% of the isolates were obtained by direct plating, with 15.6% being pure cultures. The enrichment method with reduced cultivation time yielded 72.7% of Campylobacter isolates, with 51.5% being pure cultures.A CRYOBANK for Campylobacter spp. was created to preserve genetic material and study foodborne pathogens, their properties, adaptation, resistance, and variability within the “One Health” concept. It was established that the optimal way to restore the metabolic processes of Campylobacter from a cryogenic state involves reducing the cultivation time to 24 hours during enrichment and isolation. The validation of Campylobacter isolation protocols has simplified the research process, reduced costs by 50%, and decreased laboratory workload, which will enhance the efficiency of routine Campylobacter diagnosis and identification in laboratories.
Keywords | Foodborne zoonoses of Ukraine, Campylobacter spp. isolates, Campylobacter isolation methods, CRYOBANK, Identification
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