Extracts from unripe and ripened peels of Citrus limon reveal variation in composition of bioactive compounds and exhibit antibacterial activity in relation to different extraction solvents
Extracts from unripe and ripened peels of Citrus limon reveal variation in composition of bioactive compounds and exhibit antibacterial activity in relation to different extraction solvents
TAHIR MEHMOOD*, ANUM AFZAL, ABDUL KARIM, MUHAMMAD ASHRAF SHAHEEN &
HINA ARSHAD
ABSTRACT
The goal of present study was to appraise the bioactivity of compounds using TFC (total flavonoid content), DPPH scavenging activity, reducing power assay and ABTS (radical scavenging activity) of different solvent extracts, from Citrus limon (unripe and ripened) peels. In unripe peels the total flavonoid contents, DPPH radical scavenging activity, ABTS radical scavenging activity and reducing power ranged from 18.23-57.56 mg/g CE (catechin equivalent), 21.69-65.82%, 38.96-84.83% and 0.01-1.8 mg /mL, respectively. For ripened lemon peels all these indicators ranged from 10.53-47.88 mg/g CE, 37.99-81.40%, 42.96-95.93% and 0.07-1.47 mg /mL respectively. Lemon peel extracts exhibited good antimicrobial activity against Escherichia coli, Bacillus subtilus, Staphylococcus aureus and Salmonella typhimurium. Overall ripened lemon peel extracts showed higher antioxidant activity than unripe peels. The data presented in present study is an important factor to select ripened lemon peels as high potential values for nutraceutical, pharmaceutical and cosmetic industries.
Key Words: Citrus limon, Extraction solvent system, Antioxidant activity, Antimicrobial activity
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December 2017
Vol.63, Iss. 2, Pages 125-234
