Molecular Identification of Helicobacter Pylori in Local Goats from Selected Regions in Central Iraq
Molecular Identification of Helicobacter Pylori in Local Goats from Selected Regions in Central Iraq
Ahmed Hamzah Mosa*, Hamed A. H. Aljabory, Ali Hamid. M. H. Rabeea, Lina Shaheed Waheed
ABSTRACT
The aim of the current study was to detect and examine Helicobacter pylori in goats from several districts in Iraq. A total of 60 goats from the central regions of Iraq were sampled between October 2021 and March 2022. Milk and fecal samples from all animals were analyzed for H. pylori using bacteriological culture techniques and PCR for the identification of the glmM gene (320 bp) and 16S rRNA (500 bp). H. pylori was not isolated by bacteriological culture. Of the 60 samples that were subjected to PCR analysis, 20% (12/60) and 16.6% (10/60) were positive for glmM, and 16S rRNA, respectively. The 16S rRNA gene was detected in all goats, which were also positive for the glmM gene. There was no statistically significant difference in H. pylori infection based on sex, but a significant difference was observed between age groups. Partial genetic analysis of the glmM and 16S rRNA genes from two positive samples (accession Nos. OM935719.1 and ON567364.1) showed a strong correlation with global isolates recorded in NCBI. A genetic tree was used to distinguish the glmM and 16S rRNA genes between two H. pylori strains identified from different regions in Iraq and to assess their association with global isolates. Overall, the results showed that the percentage of H. pylori infection in some governorates of the Middle Euphrates in Iraq is lower than in neighboring countries.
Keywords | glmM gene, H. pylori, Goats, PCR, Iraq
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