Molecular and Serological Detection of Toxoplasma gondii in South China Tiger
Molecular and Serological Detection of Toxoplasma gondii in South China Tiger
Yingying Wang, Jianyi Yu and Lichen Zhou*
ABSTRACT
Toxoplasmosis is one of the most important zoonotic diseases with serious health risks for wild animals. The South China tiger, the most endangered tiger subspecies endemic to China, remain only a little more than 200 today, was susceptibly infected with Toxoplasma gondii. In this study, we used polymerase chain reaction to detect DNA, and enzyme-linked immunosorbent assay to test for T. gondii antibodies. Antibodies (S/P>0.31) to T. gondii were found in 20 (26.32%) of the 76 tigers, while all blood samples tested through nested PCR were negative. This is the first investigation of T. gondii infection in South China tiger.
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