Screening for SNPs in MSTN and IGF-2 genes and its Relationship with Body Weight and Carcass Traits in Black Bronze Turkey
Screening for SNPs in MSTN and IGF-2 genes and its Relationship with Body Weight and Carcass Traits in Black Bronze Turkey
Heba Abdo Basha1, Walaa Slouma Hamouda Abd El Naby2*, Samya Erian Ibrahim3, Abeer Fikry Elnahas2
ABSTRACT
This study was conducted to evaluate the relationship between variant body weight and carcass traits of Black Bronze turkey with the single nucleotide polymorphism in the coding region of the MSTN gene and IGF-2 (3’-UTR), using DNA sequencing. Fifty turkeys were categorized into high and low body weight. Blood samples were collected from each bird for DNA extraction. Four birds were selected randomly/group to be slaughtered for carcass traits. In MSTN, only six non-synonymous SNPs were recorded leading to four amino acid substitution in exon 1 and 2 amino acids substitution in exon 2. In low body weight, only one non-synonymous mutation was recorded at exon 2. The 3’UTR-IGF-2 sequence showed 14 SNPs in high body weight and three SNPs in low body weight. The carcass weight percentage didn’t change significantly for high or low body weight. While carcass cuts percentage increased in high body weight birds compared to low weight. We conclude that no association was present between the SNPs recorded neither at MSTN coding region nor at IGF-2-3’UTR and the body weight or carcass traits. Further studies are needed to associate these SNPs with different growth stages of turkey.
Keywords | Black Bronze turkey; MSTN; IGF-2; SNPs; Body weight
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