Molecular detection and phylogenetic analysis of sheep pox virus in El Menofiya Governorate
Molecular detection and phylogenetic analysis of sheep pox virus in El Menofiya Governorate
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
ABSTRACT
Background: Sheep pox virus is a member of the Capripoxvirus genus of the Poxviridae family,
which affect sheep and causes significant economic losses in El Menofiya Governorate.
Objectives: The present study was applied for isolation and molecular identification of Sheep pox
virus from clinically affected sheep in El Menofiya governorate, Egypt during 2016. Additionally, the
isolated agent was identified using electron microscopy (EM).
Methods: Thirty five skin lesions of nodular and crusted scab samples were collected from clinically
infected SPV sheep and were prepared and isolated on chorioallantoic membrane of 9-11 days old
emberyonted chicken egg for three passages. The isolated samples were identified by EM and PCR.
Results: Five samples were positive and showed a typical pock lesion., the isolated strain was
identified as a sheep pox virus by EM and PCR. The virus was confirmed through sequence and
phylogenetic analysis of the RPO30 gene and open reading frame (ORF) 103 genes. The sequences
designated Sheep pox virus was submitted to GenBank under accession number of MF443334.
Conclusion: The molecular techniques based on the RPO30 and ORF 103 genes are efficient for the
characterization and differentiation of Capripox viruses. This finding may provide new information on
the epidemiology of SPV in El Menofiya governorate.
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January 2018
Vol. 3, Iss. 1, Pages 1-77
