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Anti-A and Anti-B Titer Among Blood Group O Donors at a Tertiary Care Centre Lahore, Pakistan

Anti-A and Anti-B Titer Among Blood Group O Donors at a Tertiary Care Centre Lahore, Pakistan

Anum Syyam1,2, Nazish Saqlain1, Sidra Hareem1, Naghmana Mazhar1, Liaqat Ali3 and Samia Afzal2*

1Department of Hematology and Transfusion Medicine, The Children’s Hospital and the Institute of Child Health, Ferozepur Road, Lahore, Pakistan.
2Division of Molecular Virology and Infectious Diseases, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
3Department of Biological Sciences, National University of Medical Sciences (NUMS) Rawalpindi.
 
* Corresponding author: samiaraza@live.com

ABSTRACT

The aim of this study was to evaluate the prevalence of IgM and IgG type anti-A and anti-B antibodies and the titer of these antibodies among blood group O donors at a tertiary care hospital. This is a cross-sectional study conducted at the department of Hematology and Transfusion Medicine in Children’s Hospital and the Institute of Child Health, Lahore, Pakistan over a period of four months from Oct, 2019 to Feb, 2020. Blood samples were collected from 350 healthy and voluntary group “O” donors (males = 320 and females = 30, median age 27 years ± 6.4 SD). Estimation of IgM type anti-A and anti-B titers was done using the standard tube technique and the samples positive for hemolysis were further evaluated for titers of IgG type anti-A and anti-B. All the titers were confirmed microscopically. The total prevalence of IgG anti-A and anti-B hemolysins was 15.9%. The most frequently observed IgM anti-A and anti-B titers were 64 in 29.4% (n = 103) and 32 in 24.9% (n = 87) donor samples, respectively. IgG type anti-A and anti-B with hemolytic activity were present in 10.8% and 5.1% donor samples, respectively. IgG type anti-A and anti-B alone were observed in 9.1% and 3.4% donor samples, respectively while both IgG type anti-A and anti-B were observed in 1.7% donor samples. Gender, age and Rh blood group did not have any significant impact on anti-A and anti-B titer frequencies. To conclude IgM and IgG anti-A and anti-B antibodies exist in significant frequencies and titers among group O donors in Lahore. It is recommended that the transfusion of group O blood to non-O recipients should be done after evaluating the titer of anti-A and anti-B hemolysins.

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Pakistan Journal of Zoology

December

Vol. 54, Iss. 6, Pages 2501-3000

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