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Fecal Antibiotic Resistome of Pigs from a Small-Scale Piggery in Ibadan, South-West Nigeria

Fecal Antibiotic Resistome of Pigs from a Small-Scale Piggery in Ibadan, South-West Nigeria

Oluseyi A. Olayinka1, Temitope O.C. Faleye2, Oladipo O. Omotosho3, Oladapo A. Odukaye3, Bolaji Oluremi4, Ibipeju H. Ibitoye1, Oludayo O. Ope-Ewe1, Uwem E. George5, Oluwadamilola A. Arowolo6, Ijeoma M. Ifeorah7, Ewean C. Omoruyi8, Emmanuel Donbraye9, Olufunke P. Adeniji10, Olubusuyi M. Adewumi1,11* and Johnson A. Adeniji1,11,12

1Department of Virology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria; 2Center for Human Virology and Genomics, Department of Microbiology, Nigerian Institute of Medical Research, Lagos State, Nigeria; 3Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria; 4Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria; 5Department of Biological Sciences, Redeemer’s University, Ede, Nigeria; 6Viral Vaccines Production Division, National Veterinary Research Institute, Vom, Plateau State, Nigeria; 7Department of Medical Laboratory Sciences, Faculty of Health Sciences and Technology, University of Nigeria, Nsukka, Nigeria; 8Institute of Child Health, College of Medicine, University of Ibadan, Ibadan, Nigeria; 9Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, Ile-Ife, Nigeria; 10Department of Transport and Tourism, Redeemers University, Ede, Nigeria; 11Infectious Diseases Institute, College of Medicine, University of Ibadan; 12WHO National Polio Laboratory, University of Ibadan, Ibadan, Nigeria.


This study was designed to sample the fecal Resistome of Pigs from a small-scale Piggery in Ibadan, South-West Nigeria. Three fecal pellets were randomly picked from the floor of unit pens containing a minimum of three pigs per unit in selected piggery in Ibadan, Nigeria. The samples were pooled and resuspended in phosphate buffered saline. The suspension was then subjected to nucleic acid extraction, cDNA synthesis and Illumina sequencing. Antibiotic Resistance Genes (ARGs) in the raw reads were determined and assembled using the Kmer Resistance tool v2.2. From the 2,974,257 reads generated, 21 ARGs with statistically significant reads were identified. Almost all targeted broad-spectrum antibiotics with over 50% targeting Tetracyclines. Five (ant(6)-Ia_3, tet(40)_1, tet(Q)_1, tet(W)_5 and tet(O/W)_4) of the ARGs were predicted to be plasmid-borne. Our findings show that the Swine industry in the region might be both a mixing pot and reservoir of ARGs. It is therefore crucial that effort is made to educate the stakeholders on the importance of good antibiotics stewardship.

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Veterinary Sciences: Research and Reviews


Vol. 9, Iss. 1, Pages 1-86


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