Submit or Track your Manuscript LOG-IN

Distribution of Antibiotic Resistance and Antibiotic Resistant Genes in Campylobacter jejuni Isolated from Poultry in North West of Pakistan

Distribution of Antibiotic Resistance and Antibiotic Resistant Genes in Campylobacter jejuni Isolated from Poultry in North West of Pakistan

Sher Bahadar Khan1, Mumtaz Ali Khan2,*, Hameed Ullah Khan3, Sher Ali Khan4, Shah Fahad5, Faheem Ahmad Khan6, Irshad Ahmad7, Nighat Nawaz8, Sidra Bibi9 and Muhammad Muneeb10

1Department of Animal Health, The University of Agriculture, Peshawar, Pakistan
2Department of Livestock and Dairy Development, Govt. of Khyber Pakhtunkhwa, Peshawar, Pakistan
3Veterinary Research Institute, Peshawar, Pakistan
4Direcotorate General of Agriculture Research, Peshawar, Pakistan
5University of Swabi, Swabi, Khyber Pakhtunkhwa, Pakistan
6Centre for Biomedical Research, Key Lab of Organ Transplantation, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China
7Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan
8Department of Chemistry, Islamia College University, Peshawar, Pakistan
9Department of Poultry Sciences, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan
10Department of Food Science and Technology, Faculty of Nutrition Sciences, The University of Agriculture, Peshawar, Pakistan

*      Corresponding author: dr_mumtazkhan@yahoo.com

ABSTRACT

Campylobacter species are one of the most important food borne zoonotic pathogens. A total of 1260 poultry meat samples were collected from four different regions of Khyber Pakhtunkhwa province and processed for isolation of campylobacter species. A total of 182 (14%) Campylobacter jejuni were isolated using enrichment and plate media followed by confirmation through multiplex PCR. Isolates were tested for 15 antibiotics using disc diffusion method followed by detection of their respective antimicrobial resistant genes. Overall prevalence of Campylobacter jejuni was 14% being higher in Peshawar division (21%) followed by Bannu division (16%), Malakand division (13%) and Hazara division (8%). Over all highest antibiotic resistance was found against AMX (93%) followed by LIN (88%), AMP (86%), TET (82%), SXT (75), CHL (68%), CLR (65%), STR (50%), GEN (44%), OFX (27%), CIP (25%), LFX (13%) and AZM (11%) while the least resistance was found against GAT (8%) and CRO (9%). 90% isolates were found to have multiple drug resistance. As for as antibiotic resistant genes are concerned, the highest ARG was blaTEM (93%) followed by tetA (82%), sul2 (75%), blaSHV (72%), tetC (71%), strA/strB (50%), sul1 (49%), blaCMY2 and aadA (44%) while the least resistant gene was aadb (9%) followed by sul3 (21%) and aac(3)IV (37%). About 92% isolates were found to have multiple drug resistance genes which is a matter of great concern from human public health perspective.
 

To share on other social networks, click on P-share. What are these?

Pakistan Journal of Zoology

February

Vol. 53, Iss. 1, Pages 1-400

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe