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The Motility and Viability of Kintamani Dogs Semen in Different Cooled Transport Duration

The Motility and Viability of Kintamani Dogs Semen in Different Cooled Transport Duration

Mariana Isti Dwiningsih1, I Ketut Suatha2 and I Ketut Puja3*

1Undergraduate Student of Veterinary Medicine, Faculty of Veterinary Medicine, Udayana University, Jimbaran, Bali, Indonesia; 2Department of Veterinary Sciences, Udayana University, Denpasar, Indonesia; 3Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, 80361, Indonesia.

 
*Correspondence | I Ketut Puja, Veterinary Genetics and Reproduction Technology Laboratory, Faculty of Veterinary Medicine, Udayana University, Bali, 80361, Indonesia; Email: asubali@unud.ac.id

ABSTRACT

Various efforts have been made to conserve the Kintamani dog, and one such approach is through artificial insemination (AI). To ensure optimal results in artificial insemination, understanding the factors that influence its success is crucial. This study aimed to investigate the impact of different transport times (0 hours, 1 hour, 2 hours, and 3 hours) on the motility and viability of chilled semen on Kintamani dogs. The study used a completely randomized design, with four treatments and six replications. In experiment 1, sample examined immediately after added extender, experiment 2, 3 and 4 were. The results of the study found that the average motility percentages for semen transported at 0 hours, 1 hour, 2 hours, and 3 hours were 91.00% ± 0.89, 90.83% ± 0.75, 90.17% ± 0.75, and 89.83% ± 0.75, respectively. Similarly, the average viability percentages for the same transport times were 82.00% ± 0.89, 81.66% ± 1.03, 81.66% ± 1.03, and 80.67% ± 1.03. Although there appeared to be a decline in semen quality, statistical analysis did not reveal significant differences. In conclusion, chilled semen from Kintamani Dogs transported for up to 3 hours remains suitable for artificial insemination.

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Pakistan Journal of Zoology

June

Pakistan J. Zool., Vol. 56, Iss. 3, pp. 1001-1500

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