Phylogenetic analysis of Egyptian foot and mouth disease virus endemic strains in 2013
Phylogenetic analysis of Egyptian foot and mouth disease virus endemic strains in 2013
Nader M. Sobhy,1 Sunil K. Mor,2 Mohammed E.M. Mohammed,1
Iman M. Bastawecy,3 Hiam M. Fakhry,4 and Sagar M. Goyal2
ABSTRACT
Foot and mouth disease virus (FMDV) is a long known virus but still a current threat in Egypt with devastating consequences as shown in 2012 in Egypt. One of the most important FMD features is high potential for genetic and antigenic variation. Therefore molecular study of FMDV is pre-requisite for amplification of FMDV specific genomic fragment and also copying specific serotypes O, A and SAT2 nucleotide sequence. In this work FMDV serotype O, A and SAT2 were isolated from cattle clinical samples (foot lesions). RNA extracted from clinical samples was subjected to reverse transcriptase polymerase chain reaction( Rt. PCR) nucleotide of FMDV ID and 3D genes were determined using standard automated sequencing technique. Phylogenetic analysis of FMD / SAT-2 /3D/Egypt/Sharkia/2013/ FMD / (KJ210080) revealed (99–100%) identity with Egypt 2012/ and Palestinian-Gaza virus. Phylogenetic analysis of serotype O of this study from Ismailia (KJ210076) , Alexandria (KJ210074) and El-Mania (KJ210077) showed 100% identity with each other and 92% identities with Asian type O sequences from Pakistan, Iran and Turkey while FMD/A/3D/Egypt/Al-Fayoum /2013/KJ210072 with serotype (A) sequences had closest identity with Asian Gulf area serotypes 95% identity with Iraq and Bahrain Also 94% with Iran/05. FMDV O, A and SAT2 strains were homologous with the vaccinal bovine strain.
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