Effect of Adding Different Concentrations of Vitamin C and E to Improve Poor Semen Quality in Ram
Effect of Adding Different Concentrations of Vitamin C and E to Improve Poor Semen Quality in Ram
Sumaya Loay Mohamed Shams Al-Dean1*, Sura Shakir Hammoud2, Mohammedali J. Ghafil3
ABSTRACT
Abstract | Sperm quality is influenced by cryopreservation. In an egg-yolk citrate extender, the current study evaluates the impact of various vit Cand E concentrations and their fusion on inferior-quality Ram semen. In this investigation, Four Ghezel-Merinose rams, who were roughly two years old, were employed .They were kept in settings of uniform lighting, housing, and food. The farm’s feeding programme was applied to the experimental animals as well. Ejaculate of the rams is divided into seven groups. For the investigation, only ejaculates with a weak motile function were chosen. Divided into four equal aliquots, each ejaculate was diluted using an Egg-Yolk-Citrate (EYC) extender penicillin, streptomycin, and distilled water to make a resulting in conc. of 4×108 sperm/ml. Six duplicates of the study were conducted for both vitamins and their fusion. The standard error of the mean (SEM) is represented as the mean ±. The effects of various treatments on motion characteristics, plasma membrane integrity, and normal acrosome morphology were evaluated using paired sample T-test. The p-value (<0.05) is considered statistically significant. After thawing, the sperm cells’ motility dramatically increased with the addition of 2 mM (56.8±1.16), 4 mM (57.6±1.36), and 7 mM (62.1±1.67). The sperms viability is improved post-thawimg and at 5C after addition of anti-oxidants vitamin C. When compared to the control values, the fresh, 5C, and post-thaw sperm motility improved significantly for each concentration of vitamin E. The p-value for 2mM(0.030), 4mM(0.04), and 7mM(0.03). Based on the current findings, it is concluded that vitamin C and E are very effective antioxidants in egg-yolk citrate extender. They can also protect ram sperm during cryopreservation by reducing oxidative stress caused by thawing. Nonetheless, there aren’t any appreciable variations in normal acrosome % between the treatment and control groups. This study concludes that the quality of ram semen improves on treating with ascorbic acid and tocopherol in egg-yolk citrate extender. The addition of vitamin C and Vitamin E to the sample improves the motility, viability, and membrane integrity of poor semen quality.
Keywords | Vitamin C, E, Semen, Ram, Cryopreservation, Antioxidants
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