Screening for Pathogenic Genetic Variants of APOA1 and ABCG1 Genes Associated with Dyslipidemia in Type 2 Diabetic Patients
Screening for Pathogenic Genetic Variants of APOA1 and ABCG1 Genes Associated with Dyslipidemia in Type 2 Diabetic Patients
Asifa Majeed*, Amir Rashid, Palvasha Waheed, Asma Faryal, Aden Razaq and Ayesha Maryam
ABSTRACT
Diabetes mellitus is getting an epidemic worldwide and Pakistan stands at position 3rd with the 17% prevalence of diabetic adults. Diabetes develops several clinical complications and dyslipidemia is one among these complications. Dyslipidemia is marked as derangements in lipid metabolism and is one of the risk factors to develop cardiovascular diseases. The genetic predisposition in association with environmental and lifestyle modifications can play a central role in the progression of diabetic dyslipidemia. A cross-sectional study was designed to detect pathogenic genetic variants in APOA1 and ABCG1 genes associated with dyslipidemia in type 2 diabetic patients. A total of ninety subjects of both genders, aged between 30-70 years were randomly selected and further divided into a diabetic dyslipidemia group, a diabetic group and a healthy group. Genomic DNA was extracted from peripheral blood and subjected to the polymerase chain reaction using primers of the APOA1 gene and ABCG1 gene. The amplicons were subjected to Sanger sequencing on Automated DNA Genetic Analyzer. The sequence data was analyzed on BioEdit7.2 biological software and Basic Local Alignment Search Tool against the human genome database to find any genetic variation. It has been found that ABCG1 gene and APOA1 gene exhibited a pattern of the normal nucleotide sequence in all subjects of three groups with no pathogenic genetic variation. Thus, exon 3 of the ABCG1 gene and exons 2 and 3 of the APOA1 gene do not contribute to the onset of dyslipidemia in type 2 diabetes patients.
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