Effectiveness of Saccharomyces cerevisiae and Sulfur as Supplements in Ammoniated Citronella Waste Basal Rations on the Nutrient Digestibility, Rumen Fluid Characteristics, and Methane Production
Effectiveness of Saccharomyces cerevisiae and Sulfur as Supplements in Ammoniated Citronella Waste Basal Rations on the Nutrient Digestibility, Rumen Fluid Characteristics, and Methane Production
Putri Okta Shafura1, Mardiati Zain2*, Elihasridas2, Bima Bagaskara1, Ummi Amanah1, Laras Sukma Sucitra1, Bella Veliana Utami1, Roni Pazla2, Erpomen2, Ezi Masdia Putri3, Rusmana Wijaya Setia Ningrat2, Riris Delima Purba3, Ruslan Abdul Gopar3, Putut Suryo Negoro3
ABSTRACT
The objective of the study was to find the most suitable formulation for a waste-based ration using ammoniated citronella to increase digestibility and provide optimal supplementation of Saccharomyces cerevisiae and mineral sulfur for rumen fluid characteristics. This study employed a completely randomized design comprising four treatments with four replications, with an incubation time of 48 hours. The ration was prepared based on a 50:50 composition of forage and concentrate, including P0 (Control), P1 (0.5% S. cerevisiae), P2 (0.3% sulfur), and P3 (0.5% S. cerevisiae + 0.3% sulfur). The Tilley and Terry in-vitro methods were utilized to measure rumen characteristics, nutrient digestibility, protozoan population, microbial protein synthesis, and methane gas production. The results revealed a significant impact (P < 0.05) of these treatments on nutrient digestibility and rumen fermentation characteristics. The combination of 0.5% S. cerevisiae and 0.3% sulfur tended to increase nutrient digestibility, total VFA production, and microbial protein synthesis. Additionally, there was a notable difference (P > 0.05) in rumen pH. Thus, the ammoniated citronella ration supplemented with 0.5% S. cerevisiae + 0.3% sulfur tended to decrease NH3 production, protozoa population, and methane gas production. The results lead to the conclusion that ammoniated citronella rations supplemented with 0.5% S. cerevisiae + 0.3% sulfur have the potential as ruminant feed because they can increase nutrient digestibility and rumen fermentation characteristics and decrease methane gas production.
Keywords | Digestibility, Saccharomyces cerevisiae, Sulfur, Ammoniated citronella waste
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May 2024
Vol. 12, Iss. 5, pp. 802-993
