Chemical Pretreatment Method for Enhanced Production of Bioethanol from Sugarcane Bagasse using Bacillus sp.
Chemical Pretreatment Method for Enhanced Production of Bioethanol from Sugarcane Bagasse using Bacillus sp.
Nazish Manzoor1*, Alim Khan1, Muhammad Yaseen1, Abid Ullah1, Zulqarnain2 and Iftikhar Jan2
ABSTRACT
Sugarcane bagasse (SCB) is usually considered agricultural lignocellulosic biowaste attracting worldwide attention as a potential substitute for fossil fuels derived from the sugar and alcohol industry. In this study, ethanol was produced from the acid and alkaline pretreated samples of sugarcane bagasse using a fermentation process. Amylase was partially purified from the bacterial strain N1 and its activity was checked at distinct pH and temperature. The optimum pH was from 6-8 whereas the temperature was 40 °C. Saccharomyces cerevisiea was added for enhanced fermentation and ethanol production. The acid pretreated sample produced the highest ethanol (15.2%) than the alkaline. The percent production of ethanol and the amount of sugar in pretreated samples was confirmed using High-Performance Liquid Chromatography (HPLC) and sugar contents were increased in acid pretreated samples.
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