Effect of Addition of Glucose or Fructose in Three Types of Crystalloid Fluid on Spermatozoa Quality of Gaga’ Chicken from South Sulawesi, Indonesia
Effect of Addition of Glucose or Fructose in Three Types of Crystalloid Fluid on Spermatozoa Quality of Gaga’ Chicken from South Sulawesi, Indonesia
Khaeruddin1,2, Gatot Ciptadi1, Muhammad Yusuf3, Hermawansyah2, Sahiruddin3 and Sri Wahjuningsih1*
ABSTRACT
Gaga’s chicken is a genetic resource of Indonesian livestock which must be protected and preserved. The increase in the gaga chicken population can be made by artificial insemination techniques and diluents are important factors in determining the quality of chicken spermatozoa. The aim of this study was to determine the effect of the use of three types of crystallized liquids and two types of monosaccharides on the quality of Gaga chicken spermatozoa. This study used a completely randomized design with a factorial pattern with the first factor being the type of crystalloid liquid (Ringer’s lactate, Ringer’s acetate, and KA-EN 3B) supplemented with egg yolk and the second factor being the addition of sugar (control, glucose, and fructose). The semen was diluted at a ratio of 1:10 and stored at 5 ºC for 48 h. The results showed that the type of diluent treatment affected abnormal morphology, progressive motility, local motility, total motility, immotil, linearity and straightness. In general, Ringer’s acetate-yolk glucose diluent is better in maintaining the quality of Gaga’s chicken spermatozoa during storage.
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