The Study of Artificial Insemination with Double Doses at Different Times in Friesian Holstein Crossbred
The Study of Artificial Insemination with Double Doses at Different Times in Friesian Holstein Crossbred
Trinil Susilawati1*, Aulia Puspita Anugra Yekti1, Amir Firdaus2, Dinda Ayu Damayanti2, Rizki Prafitri1, Nanang Febrianto1, Kuswati1, Achadiah Rachmawati1, Sri Wahjuningsih1, Nurul Isnaini1
ABSTRACT
The reproductive performance of dairy cows in Indonesia still needs to be improved. The method of Double-dose AI can highly improve the success of AI. The materials used were 160 cows divided into 80 cows inseminated by a single dose and 80 cows inseminated with a double dose. There were two treatments in this research; T1 was AI single dose at the 8th hour after estrus onset, while T2 AI double dose at the 2nd and 8th hour after estrus onset. The Non-Return Rate (NRR 1) was observed on the next cycle after AI. If the cows showed the estrus sign, the insemination was repeated with the same treatment and then continued to observe NRR 2 on the next cycle. After two months, the pregnancy and AI failure were observed using ultrasound. A Chi Square was performed using SAS OnDemand for Academics (ODA, Cary, NC, USA). Moreover, probability values were calculated using the least significant different testing. The results showed that NRR-1 was 83.75% and 85%, while NRR-2 was 62.5% and 68.75% on T1 and T2, respectively. Furthermore, the failure of AI was mainly caused by repeat breeders, which were 28.75% on T1 and T2. It consists of 95.65% and 87.5% of normal ovaries, Corpus luteum persistent at 4.35% and 8.34%, and ovarian hypo function at 0% and 4.16% in T1 and T2, respectively. In conclusion, Artificial Insemination using the double doses method did not significantly improve the pregnancy rate of dairy cows.
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