Iron Excess Impact on Pancreatic Beta Cell Structure and Fasting Blood Glucose Levels in Male Wistar Rats (Rattus norvegicus)
Iron Excess Impact on Pancreatic Beta Cell Structure and Fasting Blood Glucose Levels in Male Wistar Rats (Rattus norvegicus)
Anisa Muthia Fakhira1, Madihah Madihah1, Susi Susanah2, Erick Khristian3, Ratu Safitri1*
ABSTRACT
The body stores excess iron in several organs, including the pancreas, where it impairs its structure and function. The aims of this study is to ascertain the iron dosage that affects changes in pancreatic beta cell structure and fasting blood glucose levels in male Wistar rats (Rattus norvegicus). The study using 7 treatment groups with 4 replications. Iron dextran (ID) was administered intravenously in three 3-day intervals, with cumulative doses of 10, 20, 30, 40, 50, and 60 mg/kg BW, whether the control injected with physiological sodium chloride solution. The results of the study showed that both the necrosis score in pancreatic beta cells and fasting blood glucose levels demonstrated a dose-response pattern, with higher values corresponding to increasing dosages. The ID dose starting from 20 mg/kg BW showed a significant increase in pancreatic beta cell necrosis of 1.6 ± 0.55 and a fasting blood glucose level of 167.40±11.45 mg/dL, classifying it as a prediabetic state, that significantly different with control (p<0.05). Thus, ID as low as 20 mg/kg BW impaired the structure and function of pancreas of male wistar rats.
Keywords | Blood glucose, Iron excess, Pancreatic beta cells, Rat (Rattus norvegicus), Pancreas, Iron dextran
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March 2024
Vol. 12, Iss. 3, pp. 392-585
