Utilizing Next-Generation Sequencing to Develop and Characterize Microsatellite Loci in Cuttlefish (Sepia pharaonis) and Cross-Amplification in Other Sepiidaes
Utilizing Next-Generation Sequencing to Develop and Characterize Microsatellite Loci in Cuttlefish (Sepia pharaonis) and Cross-Amplification in Other Sepiidaes
Liqin Liu, Maoting Wang, Zhenming Lü, Li Gong*, ChangWen Wu, Baoying Guo and Lihua Jiang
ABSTRACT
The cuttlefish Sepia pharaonis, known for its economic value, is distributed in the tropical coastal waters of the Indo-pacific region. In this study, we developed twenty-one microsatellite loci for S. pharaonis through next-generation sequencing technology. A total of 100 alleles were detected, and the number of alleles per loci ranged from 2 to 9. The observed and expected heterozygosities per loci ranged from 0.000 to 0.531 and from 0.031 to 0.751, respectively. Polymorphism information content (PIC) showed that six loci were highly informative (PIC > 0.5). Five loci (CL1142, CL1770, CL2683, CL3494, CL 3674) significantly deviated from the Hardy-Weinberg equilibrium after a Bonferroni correction (P < 0.05), and none of the loci showed linkage disequilibrium. In addition, these loci were cross-amplified in three closelyrelated species. Nineteen, fifteen, and thirteen loci were amplified in Sepia lycidas, Sepia esculenta and Sepiella japonica, respectively.
To share on other social networks, click on any share button. What are these?
April 2019
Vol. 51, Iss. 2, Pages 399-799
