High Occurrence Rate of Multidrug-Resistant ESBL-Producing E. coli Recovered from Table Eggs in District Peshawar, Pakistan
High Occurrence Rate of Multidrug-Resistant ESBL-Producing E. coli Recovered from Table Eggs in District Peshawar, Pakistan
Tasbihullah1, Sadeeq ur Rahman2,*, Tariq Ali3 , Umer Saddique1, Shakoor Ahmad1, Muhammad Shafiq4, Sultan Ayaz2, Hamayun Khan1, Ijaz Ahmad1 Asadullah2, Raheela Taj5, Hafiz Nidaullah6 and Alamgir Khan7
ABSTRACT
Whilst food-producing-animals could be a pool of resistance-conferring elements in the existing animal production system in Pakistan, the issue has not yet judiciously been highlighted. This study was conducted to determine incidence of extended spectrum β lactamase (ESBL) - producing Escherichia coli in table eggs and human with history of close association with table eggs. For this purpose, a total of 200 table eggs and 50 stool samples from human were analyzed. Results showed that out of 80 E. coli isolates recovered from eggs, 20 (25%) were found to be ESBL-producers, while, of the 17 human-isolates, 4 (23.5%) were ESBL producers. PCR revealed that blaCTXM (blaCTXM-1=15 and blaCTXM-9=4) was carried by all 22 (91.6%) ESBL-producers with additional blaSHV2 (n=12) and blaNDM-1 (n=3), but no blaTEM was identified. A predominant combination of blaCTXM + blaSHV2 (n=12) followed by blaCTXM + blaNDM-1 (n=3) was determined. All these isolates (phylogroup D=14/24, A= 6/24 and B2=4/24) were found to be multidrug resistant displaying resistance against at least three different classes of antibiotics. Class 1 integron was carried by (21/24) followed by additional class 2 integron (15/24). A total of 8 isolates were harboring insertion sequence common region 1 (ISCR1), which was found to be linked with blaCTXM in 50% (4/8) isolates. Results of the current study indicate contamination of eggs with ESBL-producing E. coli suggesting to improve hygienic process for end consumer during- egg production.
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August 2020
Vol. 52, Iss. 4, Pages 1225-1630
