Association of PRL/XbaI Polymorphisms with Reproductive Performances in Vietnamese BT and TB Duck Lines
Association of PRL/XbaI Polymorphisms with Reproductive Performances in Vietnamese BT and TB Duck Lines
Tan Loi Le1, Thi Tuong Vi Trang2, Tuan Thanh Hoang3, Pin-Chi Tang1,4*, Ngoc Tan Nguyen2*
ABSTRACT
The prolactin (PRL) gene polymorphisms in the intron 1 region and their association with reproductive traits in the reciprocal TB and BT duck lines were assessed. The 441 bp segment of the PRL gene was amplified from the genomic DNA samples derived from total of 225 ducks (111 TB and 114 BT), and the PCR-RFLP technique to examine the genetic variations in the PRL gene among individuals. The restriction enzyme, XbaI, was digested to cleave the 441-bp PCR products and revealed the TT, TG, and GG genotypes. The TT genotype was most prevalent, with frequencies of 0.820 in TB and 0.754 in BT lines. Allele frequencies of T and G were 0.906 and 0.094 in TB, and 0.868 and 0.132 in BT, respectively. The effective population size (Ne) was 1.205 in TB and 1.297 in BT, while expected heterozygosity (He) values were 0.170 in TB and 0.229 in BT, with polymorphic information content (PIC) values at 0.203 and 0.229, respectively. Ducks with the TT/XbaI genotype exhibited significantly higher (P<0.05) egg weight compared to those with the TG/XbaI genotype in both lines. In the BT line, eggs from TT genotype ducks weighed 76.4 grams versus 74.3 grams from TG genotype ducks (P<0.05). In the TB line, TT genotype ducks had significantly higher egg weight compared to for TG genotype ducks (72.4 grams v.s 69.9 grams; P < 0.05). Furthermore, the TT/XbaI genotype ducks in the BT line (76.4 grams) showed significantly higher (P<0.01) egg weight than those in the TB line (72.4 grams). Polymorphisms were identified at the PRL/XbaI locus, with the TT genotype dominant in the population. Female ducks with the TT genotype showed higher egg weights, indicating that the polymorphism at PRL/XbaI locus may serve as a potential selection genetic marker to improve egg production. Further study is required to explore the impact of this polymorphism on efficacy of breeding selection and application. In addition, maintaining the T allele and TT genotype can be considered in practical conditions for breeding program selections to enhance the egg yield.
Keywords | Climate change, Duck, Egg production, PCR-RFLP, Prolactin gene, Reciprocal crossbred
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March 2025
Adv. Anim. Vet. Sci., Vol. 13, Iss. 3,
