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Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State

Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State

Samira M. Bolis1, Magda Mahmoud2, Salah E. Gumma3, Naser E. Bilal1 and
Isam ElKhidir4

ABSTRACT

Background: Focal epithelial hyperplasia (FEH) is a common oral infection which is usually missed
in the diagnosis because it is symptomless and its lesions can regress by time. On the other hand there
is no cell culture for Human papilloma viruses (HPVs).
Objective: Detection of HPV genotypes assocciated with FEH in childern aged three to fourteen years
old.
Methods: Fourty seven biopsies and oral smears were collected from Children (3 -14) years old, who
attended the pediatric dental clinics of Faculty of Dentistry, University of Khartoum and Khartoum
Dental Teaching Hospital, clinically diagnosed by the dental clincian as FEH cases and their guardian
agreed to participate. The study used the multiplex polymerase chain reaction (PCR) to detect the most
common HPV genotypes associated with FEH in children, beside the Papanicolaou (Pap.) stain to
show the cytological features which are usually seen in FEH. Results were analyzed using Statistical
Package for Social Science (SPSS) version 16. The Chi-Square test tested the significance difference
between the HPV genotypes PCR findings and the Pap. smears. Figures were constructed using
Microsoft Exel 2007.
Results: Eleven specimens 23.4% (11/47) were positive for the low risk genotypes. Twenty specimens
42.6% (20/47) were positive for genotypes 13 and 32. None of genotypes 18, 39, 56 and 59 was
detected. Twenty seven specimens 57.4% (27/47) were positive for the high risk genotypes.
Cytologically twenty nine samples 80.5% (29/36) which were positive for the PCR showed
koilocytosis and mitosoid cells. Some specimens were positive for more than one genotype. FEH was
more common in females than males. There was a significant difference between the PCR/Pap.
findings.
Conclusion: The most frequent genotypes which were detected were HPV 32 followed by HPV 16.
HPV genotypes 31, 33, 35, 52, 58 and 66 were detected in this study although they were not mentioned
in the studies conducted in FEH. Pap. Stain's and PCR sensitivity was 80.5% while the specificity was
40%.

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Journal of Virological Sciences

July

Vol. 3, Iss. 1

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