Phytochemical screening and evaluation of antioxidant, antimicrobial, cytotoxic and dermal irritant activities of Ranunculus laetus extracts
Phytochemical screening and evaluation of antioxidant, antimicrobial, cytotoxic and dermal irritant activities of Ranunculus laetus extracts
ZAMARA MASOOD1, FAROOQ SALEEM1, SARFRAZ AHMAD2, TALHA JAMSHAID3, IMRAN WAHEED4,
MUHAMMAD AZEEM1, UMAR FAROOQ GOHAR5 & HAFIZ MUHAMMAD ARSALAN6, 7*
ABSTRACT
In present study, different leaves, roots and floral extracts i.e. methanol,
chloroform, and n-hexane of Ranunculus laetus (R. laetus) were tested to
find out their bioactivities. The yield obtained from the methanolic extracts
was 15.12%, 20.5% and 31.3%, while that of chloroform extracts 4.15%,
16.15% and 29.6% and that of n-hexane extract 4.52%, 10.55% and
31.8%. The established protocols were carried out with slight
modifications for phytochemical and pharmacological activities.
Phytochemical screening revealed the presence of carbohydrates,
glycosides, cardiac glycosides, saponins, flavonoids, cumarins, steroids,
proteins, and amino acids, fixed fats and oils in all fractions. Floral
extracts were found to contain highest total phenolic contents (64 to 73
mg GAE/g), whereas leaves n-hexane fraction exhibited maximum DPPH
potential (70 %). All the extracts showed promising antimicrobial activity
in terms of zone of inhibition against selected bacterial strains, both Gram
positive and Gram negative i.e. from 1.33 mm to 25.66 mm and against
fungal strains i.e. from 1.33 mm to 24.33 mm. These findings suggest that
the different parts of R. laetus exhibiting different biological activities and
could be used as a promising source of novel drugs.
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June 2020
Vol.66, Iss. 1, Pages 1-126
