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Laboratory experiments on microbial control of Pityogenes chalcographus L. (Col. scolytidae) with entomopathogenic fungi

Laboratory experiments on microbial control of Pityogenes chalcographus L. (Col. scolytidae) with entomopathogenic fungi

G. A. Bajwa, R. Kehr and G. Zimmermann

ABSTRACT

The entomogenous fungi Beauveria bassiana (Bals) Vuill. (Bba 3), Metarhizium anisopliae (Metch) Sorok, (Ma 3, Ma 6 and Ma 43), Paecilomyces fumosoroseus (Wize) Brown et Smith (Pfr 11) and P. cicadae (Miquel) Samson (Pc 3) were tested in the laboratory for virulence on Pityogenes chalcographus. 20 adults of P. chalcographus contaminated with conidia by releasing on agar plates for two minutes were reared on bark of Picea abies (4 x 4 cm). B. bassiana, M. anisopliae (Ma 3, Ma 6, Ma 43) and P. fumosoroseus caused 100 percent mortality within 10 days, while in control and P. cicadae that was 20 and 30 percent, respectively. Conidial suspensions of B. bassiana (Bba 3), M. anisopliae (Ma 6) and P. fumosoroeus (Pfr 11) in Tween 80 (0.1%) were sprayed at the concentration of 2 x 107 conidia/ml on logs (40 cm x 15-20 cm dia) of P. abies. 410 P. chalcographus adults were released in each treatment and mortality was estimated from the emergence of adults in next generation. Population in next generation was reduced in the order, B. bassiana (46.9%), M. anisopliae (15.1%) and P. fomusoroseus (1.4%).

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Pakistan Journal of Forestry

June

Vol. 74, Iss. 1

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