Male Reproductive System Cytotoxicity and Immunotoxicity Evaluation Following Folcord Exposure
Male Reproductive System Cytotoxicity and Immunotoxicity Evaluation Following Folcord Exposure
Salema Lafta Hassan1*, Taghred Jabbar Humadai1, Sabrin Ibraheem Mohsin2
ABSTRACT
In our work sought to assess the immunotoxicity of Folcord as well as the histological status of male mice testes. Eighty adult Swiss Albino mice were separated into four groups at the age of two months. The first group (G1) was given Folcord (500 mg/kg) orally daily for four weeks, whereas the second group (G2) was given Folcord as well as I/P inoculation with 0.1 ml oil adjuvant vaccine (OAV) of Pasteurella multocida twice a week for two weeks. The third group was vaccinated by I/P with 0.1 ml oil adjuvant vaccine (OAV) of Pasteurella multocida in two doses separated by two weeks. The fourth group (G4) was assigned the role of negative control at this time, the 28th day after immunization for work, serum samples were taken for passive hemagglutination test (PHA); Immunoglobulin G(IgG) detection using a chemical immunosorbent assay test. the outcomes of PHA test revealed that Folcord inhibited humoral immune response, with a considerable rise in antibody titer in the third and second groups, but decreased serum antibody levels in the first group. Furthermore, the (IgG) titer in serum is measured using the Enzyme linked immunosorbent assay (ELISA) method. G1 revealed a significant decrease (P≤0.05) in the first group when compared to G2, G3, and G4. Histopathological examination of the testes revealed testicular degeneration, together with the absence of spermatids, no epididymal sperm in the epididymal tubules. In conclusion, Folcord has a severe toxic effect on mice, as evidenced by histological and immunological changes in the male reproductive mice, according to the results of the current study.
Keywords | Spermatogenesis, Mice, Experimental design, Folcord, Oil adjuvant vaccine (OAV), Pasteurella multocida
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February 2024
Vol. 12, Iss. 2, pp. 194-391
