Evaluation of Tip Culture and Thermotherapy for Elimination of Carnation Latent Virus and Carnation Vein Mottle Virus from Carnation Plants
Evaluation of Tip Culture and Thermotherapy for Elimination of Carnation Latent Virus and Carnation Vein Mottle Virus from Carnation Plants
Ahmed*, Amal A.; Khatab*, Eman A.H. ; Dawood*, Rehab A. and Ismeil*, Amira .M.
ABSTRACT
Carnation latent virus (CLV) and Carnation Vein Mottle Virus ( CarVMV ) were of the most important virus affecting carnation plants .The two viruses were detected serologically by DAS-ELISA using specific antibodies . Shoots from plants infected with ( CLV & CarVMV ) which maintained in green house were used for tip culture . Murashige and Skoog medium (MS ) supplemented with (0.2 mg/L BA ) and (Img/l BA and 0.5 mg/L kinetin ) were used for proliferation and micropropagation of the infected shoot clumps respectively. The plantlets were rooted in MS medium supplemented with (1.5 mg/l NAA ) . Plantlets regenerated from meristem measuring 0.1 mm and 0.2 mm yielded (90 and 80 % ) for CLV and (92 and 80 ) for CarVMV virus-free tested plantlets respectively,while larger meristems (0.3 and 0.4 mm ) were not effective . As well as the plantlets survival was (20 , 35 , 65 and 80% ) for meristem measuring( 0.1, 0.2, 0.3and 0.4 mm ) respectively . Where as the exposure of carnation plantlets infected with CLV and CarVMV to three temperature regimes (36 , 38 and 40±1 0c for 3 and 4 weeks ) .1t was found that (38±10c/3weeks ) gave ( 65 and 70 % ) for CLV and CarVMV virustested plantlets respectively and plantlets survival were ( 67 and 40 % ) While ( 38 and 40±1 Oc for 4 weeks ) gave higher precentage of virus —free plantlets but has a negative correlation with the survival of the plants
However , because of better survival rate , the temperature regime 38 Oc/3weeks is recomended for virus —free tested carnation plants.
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July 2010
Vol. 7, Pages 1-232
